Plant Transcription Factor Database
Previous version: v3.0
Oryza longistaminata
FAR1 Family
Species TF ID Description
AMDW01034480.1_FGP001FAR1 family protein
AMDW01035649.1_FGP001FAR1 family protein
AMDW01037255.1_FGP001FAR1 family protein
AMDW01040774.1_FGP001FAR1 family protein
KN538681.1_FGP300FAR1 family protein
KN538688.1_FGP103FAR1 family protein
KN538688.1_FGP146FAR1 family protein
KN538709.1_FGP108FAR1 family protein
KN538779.1_FGP003FAR1 family protein
KN538859.1_FGP013FAR1 family protein
KN538871.1_FGP005FAR1 family protein
KN538893.1_FGP004FAR1 family protein
KN538917.1_FGP001FAR1 family protein
KN538918.1_FGP012FAR1 family protein
KN538930.1_FGP009FAR1 family protein
KN539106.1_FGP020FAR1 family protein
KN539262.1_FGP008FAR1 family protein
KN539403.1_FGP013FAR1 family protein
KN539430.1_FGP005FAR1 family protein
KN539566.1_FGP003FAR1 family protein
KN539590.1_FGP003FAR1 family protein
KN539644.1_FGP008FAR1 family protein
KN539852.1_FGP007FAR1 family protein
KN539882.1_FGP003FAR1 family protein
KN540439.1_FGP001FAR1 family protein
KN540502.1_FGP001FAR1 family protein
KN540508.1_FGP001FAR1 family protein
KN540735.1_FGP001FAR1 family protein
KN540758.1_FGP001FAR1 family protein
KN541071.1_FGP002FAR1 family protein
KN541160.1_FGP002FAR1 family protein
KN542241.1_FGP002FAR1 family protein
KN542275.1_FGP001FAR1 family protein
KN542312.1_FGP001FAR1 family protein
KN542337.1_FGP002FAR1 family protein
KN542505.1_FGP002FAR1 family protein
KN542584.1_FGP001FAR1 family protein
KN542610.1_FGP002FAR1 family protein
FAR1 Family Introduction

We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.

We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the "a" fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5'-TTCACGCGCC-3'. Mutating the core sequence "CACGCGC" of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, "CACGCGC" likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H.
Transposase-derived transcription factors regulate light signaling in Arabidopsis.
Science, 2007. 318(5854): p. 1302-5.
PMID: 18033885